Promoter recognition and promoter strength in the Escherichia coli system.

The effect of promoter strength, supercoiling and secondary structure on mutation rates in Escherichia coli.

Four mutations resulting in opal stop codons were individually engineered into a plasmid-borne chloramphenicol-resistance (cat) gene driven by the lac promoter. These four mutations were located at different sites in secondary structures. The mutations were analysed with the computer program mfg, which predicted their relative reversion frequencies. Reversion frequencies determined experimental...

expression of reteplase in escherichia coli top10 using arabinose promoter

Tuning the Dragon Promoter Finder System for Human Promoter Recognition

Discovery of new genes through the identification of their promoters in anonymous DNA and the study of transcriptional control make promoter and transcription start site recognition an important issue for Bioinformatics. The biological process of transcription activation is very complex and is not completely understood. Hence computer systems for promoter recognition may not perform well if we ...

CadC-mediated activation of the cadBA promoter in Escherichia coli.

The transcriptional activator CadC in Escherichia coli, a member of the ToxR-like proteins, activates transcription of the cadBA operon encoding the lysine decarboxylase CadA and the lysine-cadaverine antiporter CadB. cadBA is induced under conditions of acidic external pH and exogenous lysine; anoxic conditions raise the expression level up to 10 times. To characterize the binding mechanism of...

Tuning Promoter Strength through RNA Polymerase Binding Site Design in Escherichia coli

One of the paramount goals of synthetic biology is to have the ability to tune transcriptional networks to targeted levels of expression at will. As a step in that direction, we have constructed a set of 18 unique binding sites for E. coli RNA Polymerase (RNAP) δ⁷⁰ holoenzyme, designed using a model of sequence-dependent binding energy combined with a thermodynamic model of transcription to pro...